不同产地余甘子黄酮类成分指纹图谱研究

HPLC Fingerprint Analysis of Flavonoids of Phyllanthi Fructus from Different Habitats

目的:建立不同产地余甘子黄酮类成分高效液相色谱指纹图谱。方法:采用高效液相色谱法,Agilent C18色谱柱,以0.5%甲酸水(A)-乙腈(B)为流动相进行梯度洗脱,在254 nm波长下对10个不同产地的余甘子药材黄酮类成分进行测定,建立余甘子黄酮类成分的HPLC指纹图谱,对其品质进行评价。结果:建立了余甘子黄酮类成分指纹图谱,10个不同产地余甘子黄酮类成分有18个共有峰,除广西、广东产余甘子外,其他产地18种黄酮类成分指纹图谱相似度均在0.90以上。结论:该方法简便、准确、重复性好,可为余甘子药材有效成分的品质评价及其质控标准的制定提供科学依据。

Objective： To establish the HPLC fingerprint of flavonoids of Phyllanthi Fructus from different habitats. Methods： HPLC method was adopted. The flavonoids composition of Phyllanthi Fructus from 10 different habitats was determined on an Agilent C18 chromatographic column with 0. 5% formic acid water（ A）-acetonitrile（ B） as the mobile phase in gradient elution under the wavelength of254 nm. The HPLC fingerprints of flavonoids composition of Phyllanthi Fructus were established to evaluate the qualitiy of them. Results： The HPLC fingerprints of flavonoids composition of Phyllanthi Fructus from 10 different habitats were established. 18 common peaks were found and the similarities of them were more than 0. 90 except the ones from Guangxi and Guangdong. Conclusion：The method is simple,accurate and repeatable. It can be used for research and quality control of the effective components in Phyllanthi Fructus.