SiO_(2)荧光纳米复合粒子的制备及性能研究

Preparation of SiO_(2) Fluorescent Complex Nanoparticles and Study on Their Properties

采用改进的Stber水解法制备了3种复合有荧光指示剂Ru（bpy）3Cl2的SiO2荧光纳米复合粒子,利用场发射扫描电镜、X射线衍射分析和红外光谱等对其进行了表征,并研究了SiO2荧光纳米复合粒子的荧光光谱。确定了最优制备条件：T=25℃,7.0mL氨水,1.3mL正硅酸乙酯（TEOS）,1.5mg Ru（bpy）3Cl2,溶剂为乙醇。在此条件下制备的SiO2荧光纳米复合粒子形貌为规则的球形,平均粒径为400-450nm,并且在水溶液中具有很好的长期稳定性。利用其固定的葡萄糖氧化酶在高温下具有良好的稳定性。这是一种SiO2荧光纳米复合粒子绿色环保的制备方法,后处理过程简单,产物有利于在生物医学领域的应用。

Three kinds of SiO2 fluorescent complex nanoparticles combined with fluorescent indicator Ru（bpy）3Cl2were prepared by the improved St？ber hydrolysis method.The characterizations were performed by field emission scanning electron microscopy,X-ray diffraction analysis and infrared spectrum analysis.The fluorescence spectrum of SiO2 fluorescent nanocomposite particles was detected as well.The optimal preparation conditions were obtained,that is T=25 ℃,7.0mL ammonia,1.3mL ethyl orthosilicate（TEOS）,1.5mg Ru（bpy）3Cl2,and the solvent is ethanol.Under these conditions,SiO2 fluorescent complex nanoparticles with regular spherical morphology have the average particle size of 400-450nm.These compound nanoparticles have a good long-term stability in water.The immobilized GOD molecular is more stable than free enzyme at a high temperature and leads to increasing thermo stability.This is a green preparation method of SiO2 fluorescent complex nanoparticles,which is benefit to environment protection.With simple posttreatment process,the product will be advantageous for biomedical applications.