高效液相色谱法测定四味光明盐汤散中胡椒碱的含量

DETERMINATION OF PIPERINE IN SIWEI GUANGMINGYAN TANGSAN BY HPLC

目的:建立四味光明盐汤散中胡椒碱的含量测定方法。方法:高效液相色谱法。Phenomen C18色谱柱(250 mm×4.6 mm,5μm);甲醇-水(77:23)为流动相;流速为1.0 m L/min;检测波长为343nm;柱温30℃。结果:胡椒碱在50.80 ng^508.0 ng范围内与峰面积积分值呈良好的线性关系,回归方程为y=664890 x+53019,r=1.0000。低、中、高浓度平均加样回收率分别为99.31%、102.4%、102.4%,RSD%分别为0.84%、0.74%、0.45%。结论:该方法准确、精密、重复性好,可作为四味光明盐汤散中胡椒碱的含量测定方法。

Objective： To establish the determination method of Piperine in Siwei Guangmingyan Tangsan. Methods： Phenomen C18 column 250 mm ×4. 6 mm,5μm） was used in HPLC assay; mobile phase was methanol-water（ 77： 23）; detection wavelength was 343nm; flow rate was 1. 0 mL / min; column temperature was 30℃. Results： The Calibration curve of Piperine was linear in the range of 50. 80 ng - 508. 0 ng the regresstion equation was y = 664890 x ＋ 53019,r = 1. 0000,The lower,middle and high concentration of the average recovery were 99. 31%,102. 4%,and 102. 4%,RSD% were0. 84%,074%,0. 45%. Conclusion： The method was accurate,precise and had good reproducibility,which can be used as determination method of Piperine in Siwei Guangmingyan Tangsan.