摘要
目的：建立蒙药六味安消散（阿木日-6）中君药-土木香所含主要成分-异土木香内酯的含量测定方法,为蒙药六味安消散的质量控制提供科学依据。方法：采用美国Trace DSQⅡ型气相色谱仪,柱子：TR-WAX;柱箱温度：起始50℃,停留0 min,升温速率20℃/min,最终温度250℃,停留6 min,升温速率5℃/min,最终温度280℃,停留5 min;载气为氮气,流速为1 mL/min;进样口温度280℃;FID检测器,温度为300℃。结果：异土木香内酯在0.01-0.20 mg/mL范围内呈良好的线性关系,R2=0.9993,平均回收率为99.52%,RSD为2.00%。结论：该方法简便、快速、准确,重现性好,可为六味安消散的质量控制提供方法。
Objective： To establish a method for the determination of iso-alantolactone in Liuwei anxiao san. Methods： GC（ Gas Chromatography） was performed on TR- WAX capillary column（ 30m×0. 25mm×0. 25μm） with nitrogen as the carrier gas. The column temperature was programmed from50℃（ holding 30min） at a rate of 20℃ / min to 250℃（ holding 6min） and then at a rate of 5℃ / min to280℃ finally. The injector temperature was 280℃. The detector temperature was set at 300℃.Results： The linear response ranges were 0. 01 - 0. 20 mg / mL（ R2= 0. 9993）（ n = 6）; the mean recoveries were 99. 52%. Conclusion： The method is simple,quick and accurate. It is suitable for quality control of this preparation.
出处
《内蒙古医科大学学报》
2014年第6期522-526,共5页
Journal of Inner Mongolia Medical University
基金
内蒙古医科大学博士启动基金项目(YKD2012BQ03)
