患丛枝病泡桐不同器官芽诱导研究

Germination from Different Organs of Witches'-broom Paulownia

以患病豫杂-1号泡桐的叶片、叶柄、茎段为材料,通过在培养基中施用不同的激素,观察材料的长芽情况,筛选出最佳外植体及最佳激素组合。实验结果表明:患病豫杂-1号泡桐芽诱导的最佳外植体是茎段,相应的培养基及激素组合为MS培养基,NAA0.3mg/L,6-BA12mg/L。此方案下的芽诱导率为30.19%。

Using leaves, leaf stalks and stems of wiches＇- broom paulownia as materials, applying different hormones in culture medi- um, observing the germination of materials, and obtaining the best exophyte and hormones combination. The results are as following： The best exophyte of germination of wiches＇- broom Yuza - 1 paulownia is stem, the best culture medium and hormone combination is MS and NAA0.3mg/L and 6 - BA12mg/L, the inductivity of shoot is 30.19%.