籼稻93-11类病斑突变体的特征研究

Characterization of Lesion Mimic Mutants From Indica Rice 93-11

水稻类病斑突变体是研究系统性抗病机理的理想材料。通过对籼稻93-11干种子进行辐射诱变,筛选获得了156株稳定遗传的水稻类病斑突变体,根据病斑表现型将其分为9类。突变体的病变组织大部分从播种后4~5周开始出现。通过体外接种试验,发现与野生型93-11相比,spl078和spl104对白叶枯病和稻瘟病的抗性增强。用荧光定量PCR技术对spl073,spl078和spl104中抗病相关基因PBZ1,POC1,PR1,PAL3和POX22.3的表达情况进行研究,结果表明,spl078和spl104中这些基因的表达水平高于野生型和其它突变体。进一步研究类病斑的产生与抗病通路标记基因之间的关系显示,这些标记基因在病斑形成过程中表达明显变化;而病斑形成后,表达相对稳定。本研究结果为进一步研究水稻广谱抗病机制和抗病育种提供了良好的实验材料。

Rice lesion mimic mutant is useful material for studying systemic resistance mechanism. 156 lesion mimic mutants were acquired through irradiating the dry seeds of indica rice 93- 11. These mutants can be divided into 9 types based on the shapes of the lesions. Most lesion mimics began to appear in 4 to 5 weeks after sowing. Two mutants spl078 and spl104 have displayed enhanced resistance to both rice blast and bacterial blight disease comparing with the wild-type 93- 11 in vivo experiment. The expression profiles of disease resistant related genes PBZ1,POC1,PR1,PAL3 and POX22. 3 in spl 073,spl078 and spl104 investigated by the real- time PCR assay showed that the genes in spl078 and spl104 were up- regulated. The relationship between the lesions formation and the expression of the disease related genes indicated that the expressions of the disease related genes were significantly changed when the lesions brought out.While they were almost stable after lesions formation. This research has provided the useful rice materials for understanding the broad- spectrum resistance to the multiple pathogens and disease resistant breeding.