水稻早衰突变体els-R7954的遗传分析和初步定位

Genetic Analysis and Mapping of a Rice Early Leaf Senescence Mutant els-R7954

为了深入研究水稻早衰机理,找到有效消除和延缓植株早衰的方法,本研究通过350Gy60Co-γ射线辐照水稻浙恢7954干种子,获得1份特异性水稻早衰突变体。观察该突变体生物学性状,发现苗期生长正常,分蘖末期叶片出现早衰现象,灌浆期整个植株枯黄,早衰现象非常明显,将其暂名为elsR7954(early leaf senescence)。遗传分析和基因定位发现,els-R7954受单隐性核基因控制,位于第2染色体SSR标记RM530和RM3774之间,距RM530约5.0c M。为进一步克隆该基因,丰富叶片早衰的分子机制奠定了一定基础,也为研究水稻早衰,最终提高产量和品质提供可能。

To further find an effective way of delaying or resolving the rice premature senescence,we isolated an early leaf senescence mutant from ‘Zhehui7954 ＇with 350 Gy gamma ray irradiation. It was investigated that the mutant,named els-R7954,kept normal at seedling stage but leaves showed etiolating at tiuer stage,and the whole plant withering at the later filling stage. Genetic analysis and gene mapping suggested that this trait was controlled by a single locus which was preliminarily mapped between SSR marker RM530 and RM3774 on chromosome 2,with a distance of 5. 0c M from RM530. This study not only lays a foundation for els gene cloning and explanation of molecular mechanism of leaf senescence,but also provides a possibility of improving the yield and quality of rice.