菌质可溶性膳食纤维对肠道菌群体外生长的影响

Effect of Soluble Dietary Fiber of Fungal Substance on Growth of Intestinal Microbiota in Vitro

研究菌质可溶性膳食纤维对肠道菌群体外生长的作用效果,为其在保健食品中的应用提供参考依据。体外试验分为空白对照组和菌质可溶性膳食纤维组的低、中、高浓度组(质量浓度分别为0.6,1.2,2.4 g/100 m L)。用菌落计数法和菌落大小法观察菌质可溶性膳食纤维对大肠杆菌、金黄色葡萄球菌、白色假丝酵母、保加利亚乳杆菌、长双歧杆菌体外生长的影响。结果表明:不同浓度的菌质可溶性膳食纤维对大肠杆菌、金黄色葡萄球菌、白色假丝酵母均有显著的抑制作用(P﹤0.05或P﹤0.01),对保加利亚乳杆菌和长双歧杆菌均有显著的促进作用(P﹤0.05或P﹤0.01),且抑菌效果和促生长效果均随着菌质可溶性膳食纤维浓度的增加而增强。菌质可溶性膳食纤维具有抑制肠道供试有害菌生长和促进肠道供试有益菌生长的作用。

To study the effect of soluble dietary fiber of fungal substance on growth of intestinal microbiota, and it would be offer the theoretical basis for its application in the production of health food. The in vitro experiment was divided into control group and different concentrations of fungal substance soluble dietary fiber（0.6, 1.2 and 2.4 g/100 m L）group. The effect of the soluble dietary fibre level on microbial activity was evaluated by colony counting method and colony size detecting method, the experimental bacterium included Escherichia coli, Staphylococcus aureus Rosenbach,Monilia albican, Lactobacillus bulgaricus and Bifidobacteriumlongum in vitro. The results showed that fungal substance soluble dietary fiber with different concentration exhibit high antimicrobial activities to Escherichia coli, Staphylococcus aureus Rosenbach and Monilia albican（P〈0.05 or P〈0.01）, and high growth promoting activities to both bulgaricus and Bifidobacteriumlongum（P〈0.05 or P〈0.01）, and its antimicrobial effect and promoting growth effect increased significantly with the concentration of soluble dietary fiber enhanced. Therefore, fungal substance soluble dietary fiber can inhibit the growth of harmful intestinal bacterium and promote the growth of beneficial intestinal bacterium in the experiment.