摘要
目的建立高效液相色谱法测定窝儿七中鬼臼毒素和4＇-去甲基鬼臼毒素的含量。方法色谱柱为COSMOSIL-C18（4.6 mm×250 mm,5μm）,柱温25℃;以甲醇为流动相A,0.4%磷酸溶液为流动相B进行梯度洗脱;流速1 m L·min^-1;检测波长330 nm。结果 4＇-去甲基鬼臼毒素、鬼臼毒素的线性范围分别为4.5-224.0μg·m L^-1（r=0.999 2）和20.0-1 000.0μg·m L^-1（r=0.999 4）,平均回收率分别为99.8%（RSD=1.5%）和99.8%（RSD=1.7%）。结论本方法灵敏、准确、重复性好,为窝儿七的质量控制提供参考依据。
Objective To determine the content of podophyllotoxin and 4＇-demethylpodophyllotoxin in Diphylleia sinensis. Li by HPLC. Methods The COSMOSIL-C18 column（4.6 mm×250 mm, 5 μm） was used. The column temperature was 25℃. Methanol-0.4% glacial acetic acid solution was used as the mobile phase at 1 m L·min^- 1. The detection wavelength was 330 nm. Results The linearity of 4＇-demethylpodophyllotoxin and podophyllotoxin was 4.5-224.0 μg·m L^-1（r = 0.999 2） and 20.0- 1 000.0 μg·m L^-1（ r = 0.999 4）, and the average recoveries were 99.8%（RSD= 1.5%） and 99.8%（RSD = 1.7%）. Conclusion The method is sensitive, accurate and reproducible. It can be used for the determination of main compositions in Diphylleia sinensis. Li.
出处
《中南药学》
CAS
2015年第2期169-172,共4页
Central South Pharmacy
基金
陕西省中医管理局科研项目(No.zy09)
