玉米YABBY转录因子家族的特征及表达分析

Characteristics and Expression Analysis of Transcription Factor YABBY Family in Maize

YABBY基因家族是广泛存在于种子植物中的一类转录因子,参与植物的胚胎发育、侧生器官分化与逆境应答等生物学过程。玉米YABBY转录因子家族含有13个成员,其N端均为锌指结构域,C端为螺旋-转角-螺旋结构域。qRT-PCR表明YABBY家族具有明显的组织特异性,除了Zm YAB1不在雌穗表达外,其余成员在生殖器官中的表达量均明显高于营养器官。Zm YAB5、Zm YAB7、Zm YAB9、Zm YAB13基因整体表达水平比较高,而Zm YAB3、Zm YAB4、Zm YAB8基因则几乎不表达。同时对玉米YABBY基因家族逆境应答反应分析发现:在4℃环境中,Zm YAB1、Zm YAB5、Zm YAB13在处理3 h内下调幅度较大,Zm YAB9在处理24 h时表达量上调;在Na Cl处理中,整个家族呈现先上调,然后逐渐下调的趋势,在PEG处理中,整个家族表达量下调。在水杨酸处理中,Zm YAB5、Zm YAB13显著上调,Zm YAB1、Zm YAB6、Zm YAB7、Zm YAB9在处理3 h内上调幅度明显,并随后回落;Zm YAB9在处理超过12 h后出现下调。ABA处理中,Zm YAB1、Zm YAB2、Zm YAB6、Zm YAB9有不同程度的上调,Zm YAB7、Zm YAB10在处理超过6 h后出现明显下调趋势,Zm YAB5、Zm YAB13整体为上调的趋势。研究结果为进一步明确该家族成员的功能奠定了基础。

YABBY gene family is a class of transcription factors widely existed in spermatophyte,and involved in plant embryonic development,lateral organ differentiation,and stress response,and other biological processes. Maize YABBY family transcription factors consisted of 13 members,containing a zinc finger-like domain in N-terminal and a helix-loop-helix domain in C-terminal. qRT-PCR showed that the expression of YABBY family presented obvious tissue specificity and was higher in reproductive organ than that in nutritive organs except Zm YAB1. Zm YAB5,Zm YAB7,Zm YAB9 and Zm YAB13 had a relatively high overall expression level,whereas Zm YAB3,Zm YAB4 and Zm YAB8 were barely. When we analyzed maize YABBY family＇s response reaction to stress,we discovered that in4℃ environment,the expression of Zm YAB1,Zm YAB5 and Zm YAB13 decreased sharply within 3 h treatment,whereas that of Zm YAB9 increased during 24 h treatment. For Na Cl treatment,the expression of whole YABBY family increased firstly and then decreased gradually. For PEG treatment,expression of the whole YABBY family decreased.For SA treatment,the expression of Zm YAB5 and Zm YAB13 increased remarkably,that of Zm YAB1,Zm YAB6,Zm YAB7 and Zm YAB9 increased significantly within 3h treatment,and then fall back,whereas that of Zm YAB9 decreased when treated over 12 h. For ABA treatment,expression of Zm YAB1,Zm YAB2,Zm YAB6 and Zm YAB9 increased with different degrees,whereas that of Zm YAB7 and Zm YAB10 showed obvious cut trend when treated over6 h. Expression of Zm YAB5 and Zm YAB13 increased for the overall trend. All these data confirm the function of all YABBY family members in maize.