补阳还五汤对BMP2/4介导轴突再髓鞘化的影响

Research to Influence of Buyang Huanwu Tang on Axonal Remyelination Mediated by BMP2/4

目的通过研究补阳还五汤对BMP2/4的干预作用,来探讨补阳还五汤对大鼠脊髓损伤后残存轴突再髓鞘化的影响。方法 将60只大鼠分为假手术组、模型组和治疗组(n=20);假手术组只打开胸10节段椎板,不损伤脊髓;模型组和治疗组采用改良Allen垂直打击法制作胸10节段脊髓损伤模型;术后治疗组给予补阳还五汤浓缩液灌胃,连续灌胃28天;分别于造模前、造模后当天、造模后7天、造模后14天、造模后28天评估每只大鼠的BBB评分,并于造模后28天取大鼠胸10节段脊髓组织,检测BMP2/4的表达变化。结果造模后28天治疗组大鼠BBB评分明显高于模型组,P<0.05(差异有统计学意义)。分析免疫印迹检测结果提示:造模后28天治疗组和模型组脊髓损伤节段BMP2/4表达量大量增加,假手术组BMP2/4少量表达,治疗组BMP2/4表达少于模型组,各组组间差异具有统计学意义(P<0.05)。结论补阳还五汤可有效抑制大鼠脊髓损伤局部BMP2/4的表达,可能通过此种途径来促进脊髓损伤后残存轴突的再髓鞘化。

Objectives To investigate the role of Buyang Huanwu Tang to axonal remyelination after spinal cord injury by studying the ef- fect of Buyang Huanwu Tang on BMP2/4. Methods 60 rats were randomly divided into three groups including sham operated group whose model approach was to only open lamina without damaging spinal cord, model group and treatment group modeling carried out in accor- dance with Allen vertical strike law, each group of 20. The rats of treatment group were gavaged with Buyang Huanwu Tang for 28 days. Results In 7 days after modeling, the BBB rating of treatment group was obvious higher than model group （P〈0.05） .And the expression of BMP2/4 of model group is most, sham operated group is least, （P〈0.05） . Conclusion Buyang Huanwu Tang could effectively inhibit the expression of BMP2/4, likely to promote axonal remyelination In this way.