鹿茸不同生长期MALAT1基因的差异表达

MALAT1 Gene Differential Expression of the Antler Tissue in Different Stages

以不同生长期的鹿茸尖端组织为材料,采用mRNA差异显示技术检测不同生长期鹿茸尖端间充质组织的差异表达基因。对其中一条差异表达片段进行克隆测序分析,得到400 bp左右的片段,与野猪的非编码基因MALAT1有高度同源性,同源性为90%。进一步实时荧光定量RT-PCR鉴定发现,该基因在鹿茸生长发育的前期和中期表达量高于后期,该基因在鹿茸快速生长期的上调表达暗示其在鹿茸生长发育过程中发挥作用,是鹿茸生长发育相关基因的候选基因。

We screened the specially expressed c DNA fragments of mesenchymal layer in different stages by mRNA differential display polymerase chain reaction（DD-PCR）.We chose a band displayed significantly differential expression to clone,check and analyze.The fragment（400 bp） had highly homology to Metastasis-associated in lung adenocarcinoma transcript1（MALAT1） with the homology of 90% to Sus scrofa＇s MALAT1.By real time RT-PCR,the expression level of MALAT1 gene in early and middle stages was higher than the expression level in late stage.The special up-regulation of MALAT1 gene in rapid growth period implied it might be an important factor in the process of growth and development of antler.