泽漆乙酸乙酯萃取物对小鼠经口急性毒性

Acute Oral Toxicity of Euphorbia helioscopia'Ethylacetate Extract in Mice

研究了泽漆(Euphorbia helioscopia)乙酸乙酯萃取物的灭鼠毒性,评价了其灌胃GHS毒性类别,并以点斜法测定其灌胃LD50和LD10。结果表明:泽漆乙酸乙酯萃取物属于GHS 3类,有较好的杀鼠活性,其灌胃LD50为314.80 mg·kg-1,95%置信区间是(314.80±38.29)mg·kg-1,最低致死剂量估计值LD10为220.53 mg·kg-1,LD10的95%置信区间是(220.53±35.61)mg·kg-1。萃取物对灌胃的KM小鼠造成了多项生理指标的显著变化,对KM小鼠心脏、肝脏、脾脏、肺脏、肾脏均造成了不同程度的损伤,并表现出多种明显的中毒症状。

With Kunming mouse as test animal,we studied Euphorbia helioscopia＇s toxicity and appraised the GHS class of E.helioscopia＇s extract by one-time intragastric administration.The intragastric administration＇s LD50 of Euphorbia helioscopia＇s ethylacetate extract.Ethylacetate extract of Euphorbia helioscopia was GHS Lever 3,and had good mouse killing active.All extracts effected moise＇s physical signs,and created obviously different poison symptoms.The intragastric administration＇s LD50 of Euphorbia helioscopia＇s ethylacetate extract was 314.80 mg·kg-1.The 95% confidence interval was（314.80±38.29） mg·kg-1.The lowest killed does estimated value was LD10 of 220.53 mg·kg-1,and at 95% confidence interval it was（220.53±35.61） mg·kg-1.Ethylacetate extract made significant changes on many physiological indexes of mouse,and injured heart,liver,spleen,lung,kidney in varying degrees.