地锦叶斑病病原菌的鉴定及其生物学特性

Pathogen Identification and Biological Characteristics of Creeper Leaf Spot

结合传统形态学和分子生物学方法对地锦（Parthenocissus tricuspidata）叶斑病病原菌进行了鉴定,并在室内纯培养条件下,采用生长速率法研究了病原菌在不同培养基、p H值、温度、光照、碳源和氮源条件下菌丝生长和菌落形态。结果显示：病原菌为马卡假尾孢（Pseudocercospora macadamiae Deighton）;病原菌生长较适培养基为PDA培养基和20%地锦植物煎汁的PDA培养基,适宜p H值为6.0~7.0,菌丝生长适宜温度范围为25~30℃,最适碳源为蔗糖,最适氮源为酵母粉,最佳光照条件为全黑暗。

We identified the leaf spot fungus of Parthenocissus tricuspidata by the means of combining the traditional morphological and molecular biological methods,and we studied the effects of medium,p H,temperature,light,carbon and nitrogen resources on mycelium growth and colony morphology by growth rate method.The pathogenic bacterium was Pseudocercospora macadamiae Deighton.The optimum medium for the mycelium growth was PDA medium and PDA medium with Parthenocissus tricuspidata extract.The optimum p H was 6-7,and the optimum temperature for mycelia growth was 25 ℃-30 ℃.The mycelium grew best on media with sucrose as carbon source,yeast as nitrogen resource and the optimum photoperiod was in darkness.