秋子梨茎段薄层细胞培养再生初探

Study on Regeneration of Thin Cell Layer of Pyrus ussuriensis

以秋子梨试管苗为试材,进行了其茎段薄层细胞组织培养再生体系的构建,以期为秋子梨无性繁殖和工厂化育苗奠定基础,同时为薄层细胞培养在梨属植物当中的应用提供参考。结果表明:秋子梨茎段薄层细胞的再生受到激素浓度和配比的影响,其中不定芽再生最佳培养基为MS+BA 1.5mg/L+NAA 0.25mg/L+蔗糖30g/L+琼脂糖7g/L,再生效率达100%;不定根再生最佳培养基为1/2MS+IBA 0.5mg/L+蔗糖30g/L+琼脂糖7g/L,根再率达66.7%;同时研究还发现适宜的暗培养有助于愈伤组织的诱导和不定芽的再生。

Regeneration system of thin cell layers（TCL）was established by using shoots of Pyrus ussuriensis as explants,to provide basis for asexual propagation and industrialized seedling production of Pyrusussuriensis,meanwhile to provide reference for application of TCL technique in Pyrusresearch.The results showed that regeneration efficiency of TCL was affected by the combination and concentration of hormones.The optimal culture medium of adventitious shoots regeneration was MS＋BA 1.5mg/L＋NAA 0.25mg/L＋sucrose 30g/L＋agarose 7g/L,and the regeneration rate was100%.The optimal culture medium of adventitious roots was 1/2MS＋IBA 0.5mg/L＋sucrose 30g/L＋agarose 7g/L,and the rate of rooting was 66.7%.Meanwhile,callus and adventitious shoots induction rate increased under dark culture.