白灵菇品种选育研究

Study on Selective Breeding of MushroomPleurotus ferulae

以"白灵0号"、"白灵1号"、"白灵2号"3种白灵菇孢子为试材,采用杂交育种及紫外诱变育种技术,在致死率75%条件下进行紫外线诱变处理;以其为亲本,采用单孢杂交(两点法)进行单核菌丝体交配后,观测菌丝的"锁状联合"、拮抗试验(三点法)和酯酶同工酶谱鉴定杂交子。通过杂交子与亲本的菌丝体生长速度、色泽及菌丝体密度对比,筛选出优势菌株26株,选育白灵菇新品种。结果表明:经过诱变处理的白灵菇孢子具有较高杂交亲和性,且杂交子呈现正向变异率高的特点。

With‘Bailing No.0＇,‘Bailing No.1＇,‘Bailing No.2＇monospore as test materials,cross breeding and UV mutation breeding technology were used to select breeding mushroomPreurotus ferulae.UV mutagenic treatment under the condition of the fatality rate of 75%.As parents,the single spore hybridization method was adopted to mononuclear mycelium after mating,Hybrids were primarily identified with clamp connection,then confirmed by antagonistic test and esterase isoenzyme analysis.The 26 strains were selected based on the comparison of mycelial growth rate,color and density among parent strain and hybrids.The results showed that Pleurotus ferulae spores treated by UV mutagenesis had high cross compatibility,and then hybrid had the characteristics of high positive mutation rate.