生鲜乳中β-内酰胺酶微生物法检测

Precautions about β-lactamase microbiological assay in raw milk

目前对生鲜乳中β-内酰胺酶的检测方法主要有高效液相色谱法和微生物检测法(杯碟法)。其中微生物检测法准确率较高,适于具有微生物检测能力的检验机构使用。本文针对微生物检测法中涉及的一些技术问题及注意事项进行整理和分析,便于相关检验人员借鉴参考。

β -lactamase is a β -lactam antibiotics inactivated enzyme in raw milk in the law forbidden to add such, Substances, can cover up the class of antibiotic residues. Detection methods for β -lactamase in raw milk present mainly HPLC and microbiological assay （plate meth- od）. Microbiological assay accuracy rate is higher, suitable for inspection agency with capability of microbial detection. In this paper, collation and analysis of some technical issues and considerations involved in microbial detection method to facilitate the relevant inspectors draw refer- ence.