低温诱导阿魏侧耳菌丝分化酶活力及同工酶的研究

Enzyme activity and isoenzymes of Pleurotus ferulae lanzi mycelium morphogenesis induced by low temperature

阿魏侧耳属中低温型食用菌,菌丝需经过低温诱导后才能形成原基。研究低温诱导前菌丝、诱导后菌丝及原基漆酶(LACC)、过氧化物酶(POD)和多酚氧化酶(PPO)活力的变化,变性聚丙烯酰胺凝胶电泳分析同工酶表达差异。结果表明:低温诱导阿魏侧耳菌丝分化过程中酶活力变化显著,低温诱导后菌丝LACC活力由150.15 U提高到290.62 U,PPO活力由0.68 U提高到1.48U;原基形成后LACC活力由290.62 U降低到3.82 U,PPO活力由1.48 U降低到0.12 U;POD活力在整个过程中呈下降趋势,由2.72 U降低到0.98 U。低温诱导对阿魏侧耳菌丝、原基蛋白质、LACC、POD和PPO同工酶表达产生了明显的影响,表现为同工酶活力差异和同工酶条带表达的差异。

Pleurotus ferulae lanzi is low medium temperature edible fungi, which low temperature was requested to the primordia formation. Studying on changes in enzyme activity of laccase（LACC）, peroxidase（POD） and polyphenol oxidase（PPO） of mycelium before hypothermia induced, mycelium after hypothermia induced and primordia and analysis the differentially expressed isozymes by denaturing polyacrylamide gel electrophoresis. The study shows that enzyme activity changes significantly after mycelium induced by hypothermia. LACC and PPO activity rises from 150.15 U to 290.62 U, and 0.68U to 1.48 U after mycelium induced by hypothermia, respectively. LACC activity decreased from 290.62 U to 3.82 U, PPO activity from 1.48 U reduced to 0.12 U after the formation of primordia. POD activity decreased in the whole process, from 2.72 U decreased to 0.98 U. Low temperature induce had a significant impact on LACC, POD and PPO isozymes and protein of Pleurotus ferulaelanzi mycelium and primordial as the differences of isoenzyme activities and isozyme banding.