高压热水浸提法提取金针菇黄酮的优化研究

Optimization of high-pressure hot water extraction of flavone from Flammulina velutiper(Fr.) Sing

研究了采用高压热水浸提法从金针菇中提取总黄酮的最适条件。通过单因素实验和正交实验,探讨了料液比、高压热水浸提时间及高压热水浸提温度对金针菇总黄酮提取率的影响,并以金针菇总黄酮提取率为评价指标,优化提取工艺。实验结果表明,高压热水浸提法提取金针菇总黄酮的最适条件为:料液比为1:40(g/m L)、高压热水浸提温度为121℃、高压热水浸提时间为60 min,在此条件下,金针菇总黄酮的提取率为12.7%。

In this paper, the optimal conditions of high-pressure hot water extraction of flavone from Flammulina velutiper （Fr.） Sing are studied. Through the single factor and orthogonal experiments, the influences of solid-liquid ratio, high pressure hot water extraction time and high pressure hot water extraction temperature on the extraction rate of flavone are discussed, and the extraction process is optimized. The experimental results show that the optimum condition is as follows： the solid-liquid ratio of 1：40 （g/mL）, high pressure hot water extraction temperature of 121 ℃, high pressure hot water extraction time of 60 min. Under this condition, flavone extraction rate is 12.7%.