摘要
建立了一种加速溶剂萃取-凝胶色谱净化-气相色谱/质谱联用法快速测定水产加工品中胆固醇氧化物含量的分析方法。采用此分析方法,测定了胆甾烯酮、7β-羟基胆固醇、5β,6β-环氧化胆固醇、5α,6α-环氧化胆固醇、20α-羟基胆固醇、3β,5α,6β-胆甾烷三醇、6-酮基胆固醇、7-酮基胆固醇等8种化合物,优化了加速溶剂萃取仪、凝胶净化色谱仪、气相色谱/质谱联用仪的条件,在10-1000 ng/m L范围内,线性相关系数可达0.9995以上;重现性良好,其相对标准偏差小于7%;回收率可达76%-103%;其灵敏度高,方法检测限低：8种胆固醇氧化物为0.1μg/kg以下,适用于水产加工品中胆固醇氧化物的检测工作。
The establishment of an accelerated solvent extraction-gas chromatography/mass spectrometry rapid determination of aquatic products of cholesterol oxide content-gel chromatography purification methods. With this method for determining the cholesteric enone, 7β-hydroxycholesterol, 5β,6β-epoxy- based cholesterol, 5α,6α-epoxy-based cholesterol, 20a-hydroxy-cholesterol, 3β,5α,6β-cholestane three alcohol, 6-keto cholesterol and 7-keto cholesterol to optimize the accelerated solvent extraction device, gel purification chromatography, gas chromatography/mass spectrometry conditions within 10-1000 ng/mL range, the linear correlation coefficient of up to 0.9995 above; good reproducibility relative standard deviation of less than 7%; recovery of up to 76%-103%; its high sensitivity, the method detection limit 8 kinds of cholesterol oxides of 0.1 μg/kg or less, to meet the requirements.
出处
《食品科技》
CAS
北大核心
2015年第3期309-314,共6页
Food Science and Technology
基金
浙江省科技计划项目(2013C37077)
质检公益性行业科研专项(201210060)
关键词
胆固醇氧化物
水产加工品
气相色谱-质谱联用
cholesterol oxidation products
aquatic products
gas chromatography-mass spectrometry
