赤芍总苷、川芎总酚酸组分组成结构对缺氧损伤人脐静脉内皮细胞的影响

Effect of different composition structures of total paeony glycoside component and total phenolic acid component of Chuanxiong Rhizome on human umbilical vein endothelial cells with hypoxic injury

目的:研究赤芍总苷、川芎总酚酸组分不同组成结构对连二亚硫酸钠(Na2S2O4)诱导的人脐静脉内皮细胞(HUVEC)缺氧损伤模型的保护作用,并参照基线等比增减法,设计不同组分组成结构,优化出最佳的组分结构。方法:采用Na2S2O4体外诱导HUVEC,建立细胞损伤模型。采用MTT比色法测定细胞活力,试剂盒测定细胞内超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、乳酸脱氢酶(LDH)含量、一氧化氮(NO)含量,通过Western blot检测了各组细胞Bcl-2,Bax蛋白的表达量。结果:与模型组比较,赤芍总苷组分、川芎总酚酸组分不同组成结构可显著提高SOD活力,降低MDA,LDH,NO含量(P<0.01,P<0.05)。芍芎组分可以下调Bax蛋白的表达量,上调Bcl-2蛋白的表达量,Bcl-2/Bax显著升高(P<0.01,P<0.05),抑制细胞凋亡。结果显示赤芍总苷、川芎总酚酸组分组成结构比例为8∶2时,对缺氧损伤的内皮细胞保护作用最好。结论:芍芎组分可以减轻HUVEC的缺氧损伤,8∶2组分组成时保护作用最为明显,其机制可能与其抗氧化和抑制细胞凋亡有关。

Objective： To study the effect of different composition structures of total paeony glycoside （TPG） component and total phenolic acid of Ligusticum chuanxiong（TLPA） on sodium dithionite （Na2S2 04 ）-induced human umbilical vein endothelial cells（ HU- VEC） hypoxic injury. The baseline geometric proportion was used to design different components structure. And then the best structure of components by cell injury model were optimized. Method： A HUVEC hypoxic injury model was established by being induced of NaES204. Cell viability was measured by MTT colorimetrie method, intracellular superoxide dismutase （SOD） activity, malondialde- hyde（ MDA）, lactate dehydrogenase（LDH） levels, nitric oxide （NO） contents were measured by kits. At last, Western blot analysis was used to detect the expression of two proteins, Bel-2 and Bax. Result： Compared with the model group, TPG component, TLPA component at different composition structures can significantly increase SOD activity and decrease MDA, LDH, NO levels （P 〈0. 01,P 〈 0. 05 ）. Paeoniae Radix Rubra and Chuanxiong Rhizoma components can downregulate the expression of Bax protein and upregulate the expression of Bcl-2 protein. The ratio of Bcl-2 and Bax was significantly increased （ P 〈 0 01, P 〈 0 05 ）, it means that cell apopto- sis was inhibited. The results indicate that among all the component composition structures, TPG and TLPA component at the propor- tion of 8：2 had the best protection on hypoxic injury of endothelial cells. Conclusion： TPG component and TLPA component can resist HUVEC hypoxia injury, the protective effect was the most evident under the structure of 8： 2, which may be due to the inhibition of in- tracellular lipid peroxidation and cell apoptosis.