肉鸡屠宰生产链中沙门氏菌耐药基因检测与耐药相关性分析

Drug resistance gene detection and the resistance correlation analysis in Salmonella isolated from broiler slaughterhouse production chain

目的:了解肉鸡屠宰生产链沙门氏菌耐药性与耐药基因的相关性。方法:采用纸片扩散法对肉鸡屠宰生产链分离鉴定的72株沙门氏菌进行10种抗生素敏感性实验;采用PCR方法检测耐药沙门氏菌相应的耐药基因。结果:72株肉鸡屠宰生产链中沙门氏菌对萘啶酸(100%)的耐药率最高;对氨苄西林、甲氧苄啶/磺胺甲恶唑、环丙沙星、四环素、庆大霉素耐药率分别为69.44%、54.17%、38.89%、34.72%、13.89%。72株沙门氏菌中有67株至少含有一种耐药基因,bla TEM、bla CMY-2、sul2、sul3、tet B和tet C耐药基因较为普遍,其耐药菌bla TEM、bla CMY-2、sul2、sul3、tet B和tet C基因检出率分别为96.08%、100%、94.87%、71.79%、78.38%和100%。结论:沙门氏菌对常见抗生素具有不同程度的耐药性,且耐药基因普遍存在于耐药菌株中,药敏实验结果与耐药基因检测结果有很高的一致性(≥75%)。

Objective：To understand the relationship between drug-resistant gene and drug resistance of Salmonella isolates from broiler slaughterhouse production chain. Method ： The susceptibility testing of 72 Salmonella strains from broiler slaughterhouse production chain to 10 varieties of antibiotics were performed by means of K-B disc method and PCR technique was used to detect the presence of resistance gene in the corresponding drug- resistant Salmonella isolated. Result：The nalidixic acid （100%）resistant rates of Salmonella isolated from broiler slaughterhouse production chain were the highest.The resistant rates of 72 Salmonella isolated to ampicilin, benzyl methyl oxygen/sulfamethoxzole, ciprofloxacin, tetracycline, gentamicin were 69.44%, 54.12%, 38.89%, 34.22% and 13.89% ,respectively.At least one antimicrobial resistance gene was detected in each Salmonella isolate and the following antimicrobial resistance genes were commonly present; the detection rates of the resistant strains of blaTEM, blaCMY- 2, suA, sub, tetB and tetC genes were 96.08%, 100%, 94.8？%, 71.79%, ？8.38%, 100%, respectively.Conclusion： These results implied that the resistant Salmonella isolated from broiler slaughterhouse production chain to common antibiotics were different from each other,and resistant genes were widely existed in these resistant strains.Antimicrobial phenotypes of Salmonella isolates were very consistent with their genotypes （ ≥75% ）.