磁性粒子表面自组装辛可宁分子印迹电致化学发光传感器

Highly Sensitive Self-assembly Magnetic Molecularly Imprinted Electrochemiluminescence Sensor for Determination of Cinchonine

以辛可宁为模板分子、十二烷基硫醇为功能单体,在Fe3O4@Au纳米粒子表面自组装辛可宁分子印迹膜,构建了新型磁性粒子-分子印迹电化学发光传感器。通过透射电子显微镜对磁性纳米粒子的粒径分布及形貌进行了表征,使用红外光谱对比分析了辛可宁、分子印迹膜洗脱前和洗脱后的结构及成分。结果表明,在最优的实验条件下（0.012 mol/L硼砂缓冲溶液（p H 9.5）,0.8 mmol/L Ru（bpy）2＋3）,辛可宁浓度的对数在1×10＂10-9×10＂8mol/L范围内,与电化学发光强度变化值有良好的线性关系,检出限为3.5×10＂11mol/L。此传感器灵敏度高、选择性好、易于更新,将其用于血清样品的检测,方法回收率为98.8%-104.7%。

A molecularly imprinted electrochemiluminescence sensor for detection of cinchonine based on surface self-assembly of molecularly imprinted membrane on magnetic nanoparticles was developed.Cinchonine was used as template and lauryl mercaptan was used as assembling monomer. The morphology and particle size distribution of the magnetic nanoparticles were characterized by TEM, and the infrared spectroscopy was used to study the structure and composition of cinchonine,molecularly imprinted membrane before and after elution. The results showed that,cinchonine could be specifically assayed with a linear relationship between the signal intensity and the logarithm of concentrations of cinchonine in the range of1 ×10^-10mol / L to 9 × 10^-8mol / L. The detection limit for cinchonine was 3. 5 × 10^-11mol / L. The sensor was used for the determination of cinchonine in serum samples,with the recoveries of 98. 8% to 104. 7%.