偏头痛患者血浆中过氧化物酶体增殖物激活受体水平的研究

Changes of serum PPARs levels in migraine patients

目的观察不同时期、不同亚型偏头痛患者血浆中过氧化物酶体增殖物激活受体(PPARs)的变化,探讨偏头痛的发病机制。方法将227例偏头痛患者分成偏头痛发作期组(A组,n=98)和发作间期组(B组,n=129);同时根据患者先兆症状的不同又分为四个亚组,即A1组-无先兆的偏头痛发作期患者(n=71),A2组-有先兆的偏头痛发作期患者(n=27);B1组-无先兆的偏头痛发作间期患者(n=94),B2组-有先兆的偏头痛发作间期患者(n=35);以年龄、性别相匹配的100例健康者作为对照组。搜集患者的临床资料(性别、年龄、BMI、血压、血脂),并采用酶联免疫法(ELISA)检测受试者血浆PPARs水平,采用SPSS系统软件进行统计分析。结果偏头痛发作期患者血浆中PPARα、PPARβ/δ水平明显高于发作间期及健康对照者的水平(572.3±68.1VS 306.3±69.2VS 301.49±74.3、591.1±72.2VS 233.1±105.6VS 245.68±101.7,P<0.05),而PPARγ水平发作期组低于发作间期组及健康对照组(157.2±104.4VS 230.3±97.2VS 221.86±95.9,P<0.05);有先兆和无先兆偏头痛发作期PPARα、PPARβ/δ、PPARγ水平与发作间期比较均有明显差异(574.9±68.5VS 308.8±70.2,588.5±70.5VS 242.7±109.7,159.8±101.6VS 233.2±96.4,P<0.05)。PPARs水平在发作间期患者组与健康对照者组之间比较无统计学显著性差异(P>0.05);无论是偏头痛发作期或发作间期,有先兆与无先兆患者血浆PPARs水平组内比较均无明显差异(P>0.05)。结论 PPARs参与偏头痛发作的病理生理过程,与有无偏头痛先兆发生无关。

Objective To observe the clinical features of patients with migraine,and analyze the plasma level of PPARs（peroxisome proliferator-activated receptors）during different periods and different subtypes in migraine patients.To explore the relationship between PPARs and migraine attacks.Methods 227 patients with migraine were divided into two main groups：attack period group（n=98）and attack free period group（n=129）.According to the patients with or without aura symptoms patients were further divided into four subgroups.100 cases of healthy were taken as control group.Collect the clinical data of patients（Gender,age,BMI,blood pressure,blood lipid.The serum levels of PPARs were measured using enzyme-linked immunoassay（ELISA）.Using SPSS software for statistical analysis.Results No significant difference about age,BMI,blood pressure,blood lipid level among migraine during headache attack period、headache free period and the control group..The PPARα、PPARβ/δlevels in patients with migraine during headache attack period were significantly higher than headache free period and healthy control group.The PPARγlevels in patients with migraine during headache attack period were significantly lower than those during headache free period and healthy control group.The PPARα、β/δ、γlevels in patients with migraine during headache attack period have significant differences compared with those during headache free period no matter with or without aura.The PPARs levels in headache free period have no significant difference to healthy control group（P〉0.05）.The level of PPARs in plasma of migraine was no significant difference between migraine with aura group and without aura group,regardless of whether during headache attack period or headache free period（P〉0.05）.Conclusion PPARα、β/δ,and PPARγinvolved in the pathogenesis of migraine.With or without aura had no obvious effect to PPARs level.