白腐真菌转化黄芩苷生成黄芩素的研究

Research on biotransformation of baicalin to baicalein by white-rot fungi

为了研究白腐真菌对黄芩苷生物的转化方法和模型,采用生长细胞转化法和静态细胞转化法对黄芩苷进行生物转化,使用超声波处理培养液,利用高效液相色谱检测转化产物.检测结果显示,在黄芩素标准品相应保留时间内有明显出峰,表明白腐真菌内的诱导酶、胞内酶能将黄芩苷转化为黄芩素.经实验确定最佳转化时间为96h,最佳pH为5.5,当料液比为1∶100时,最佳接种量是4片直径为10mm的菌饼.

The method and the model of biotransformation process of baicalin by white-rot fungi were studied.The dynamic cells and static cells were used to transform baicalin.After ultrasonic treatment on cultivation medium,the product was identified as the baicalein by HPLC.As a result,the transformation enzyme is supposed to be induced enzyme and endoenzyme at the same time.The optimal authire conditions are as follows：the reaction time is 96 h,pH is 5.5,inoculation amount is 4slices of white-rot fungi whose diameter is 10mm。