基于脂肪酸生物标记与16S rRNA的芽胞杆菌系统发育分析比较

The Comparsion of Bacillus Species Classification Based on Fatty Acid and 16S rRNA Gene

旨在探究脂肪酸作为一种有效的芽胞杆菌分类标记,以25种芽胞杆菌模式菌株为研究对象,对芽胞杆菌进行脂肪酸组分和16S rRNA基因系统进化分析比较。结果表明,脂肪酸系统发育分析能充分体现芽胞杆菌种类间的亲缘关系,并且按生物学特性进行聚类分群,而16S rRNA系统发育仅完美体现出种间的亲缘关系。利用脂肪酸分析可将25种芽胞杆菌完全准确分开,且将生物学特性相同的芽胞杆菌种类聚为一类,如碱性条件下生长良好的4种芽胞杆菌(B.agaradhaerens、B.alacalphilus、B.alkalitelluris和B.fastidiosus)聚为一类,芽胞杆菌为圆形的芽胞杆菌(B.fusiformis、B.odysseyi和B.sphaericus)聚为一类。结果表明,脂肪酸分析不仅根据亲缘关系进行聚类,还可以根据生物学特性对芽胞杆菌进行分类。

In order to explore the application of fatty acid composition in taxonomy of Bacillus species, the fatty acid constitutions of 25 strains were detected by Microbial Identification System（MIDI）. The clusters of fatty acid profiles and 16 SrRNA gene sequences were analyzed by SPPS16.0 and Mega4, respectively. The results showed that phylogeny analysis based on the fatty acid biomarkers could not only fully reflect the relationships among the Bacillus species, but also group the Bacillus species according to the biological characteristics. However, 16 S rRNA phylogeny only perfectly showed the relationships among the species. For example, four species growing well under the alkaline conditions and three species round spore-forming were clustered together, respectively. Result showed that Bacillus species can be clustered together not only according to the related ship, but also classified by the biological characteristics.