初诊恶性肿瘤患者外周血CD3^+ CD56^+ NKT样细胞的检测

CD3^+ CD56^+ NK T-like cells are significally increased in the peripheral blood of patients with newly diagnosed cancer

目的:探讨初次诊断为恶性肿瘤患者的外周血CD3^+ CD56^+ T淋巴细胞(NKT样细胞)及T调节细胞(regulatory T cells,Treg)的比率并分析其意义。方法:用流式细胞仪检测健康体检者(A组)、非恶性肿瘤患者(B组)、初诊恶性肿瘤患者Ⅰ~Ⅱ期(C组)、初诊恶性肿瘤患者Ⅲ~Ⅳ期(D组)4组患者外周血中CD4^+ CD25^(high)CD127^(low)细胞占CD4+T淋巴细胞的百分率(Treg细胞)及CD3~ +CD56^+细胞占CD3^+T淋巴细胞的百分率(NKT样细胞)。结果:A、B、C、D 4组患者外周血中Treg细胞分别为(6.72±3.16)%、(6.49±2.83)%、(6.80±3.07)%、(7.63±3.47)%,4组之间比较差异均无显著性(均P>0.05);A、B、C、D4组患者外周血中NKT样细胞分别为(3.13±1.66)%、(2.56±1.27)%、(7.22±2.70)%、(7.72±3.10)%,A、B两组患者的外周血NKT样细胞差异无显著性(P>0.05),C、D两组患者比A、B两组患者的外周血NKT样细胞明显升高(P<0.05)。结论:初次诊断为恶性肿瘤的患者外周血中NKT样细胞有明显升高的趋势。

Objective To investigate the presences of regulatory T cells （Treg） and natural killer T cell＆nbsp;（NKT）-like T cells in the peripheral blood of healthy subjects and patients with newly diagnosed cancer. Methods Patients were enrolled into four groups： healthy subjects （Group A）, patients without cancer （Group B）, patients with newly diagnosed stage Ⅰ~Ⅱ cancer （Group C）, and patients with newly diagnosed stage Ⅲ~Ⅳ cancer （Group D）. Flow cytometry was performed to detect the percentage of CD4＋ CD25high CD127low cells in CD4＋ T lymphocytes （Treg） and the percentage of CD3＋ CD56＋ cells in CD3＋ T lymphocytes （NKT-like）. Results The percentage of Treg cells in CD4＋T lymphocytes was（6.72 ± 3.16）%, （6.49 ± 2.83）%,（6.80 ± 3.07）%and （7.63 3.47）% in Group A, B, C and D, respectively, with no significant differences among these groups （P〉0.05）. However, the percentage of CD3＋CD56＋T cells in CD3＋T lymphocytes was （3.13 ± 1.66）%, （2.56 ± 1.27）%,（7.22 ± 2.70）% and （7.72 ± 3.10）% in Group A, B, C and D, respectively, with marked increases in Group C and D compared to those in Group A and B （P 〈 0.05）. Conclusion Patients with newly diagnosed cancer demonstrated increasing trends in the percentage of peripheral NKT-like cells.