甲基化转移酶抑制剂zebularine对人胃癌SGC-7901细胞株上皮性钙黏附蛋白基因表达的影响

The effect of zebularine on E-cadherin mRNA expression in SGC-7901 cell line

目的 观察甲基化转移酶抑制剂zebularine对人胃癌SGC-7901细胞株增殖、迁移和上皮性钙黏附蛋白（E-cadherin）基因表达的影响.方法 采用噻唑蓝（MTT）法和划痕实验检测zebularine对人胃癌SGC-7901细胞株的抑制率及其细胞迁移率的影响,采用实时定量聚合酶链反应（Real-time PCR）检测作用前后E-cadherin基因表达的变化.结果 （1）终质量浓度100 μmol/L的zebularine作用24h能显著抑制SGC-7901细胞株的增殖率和迁移率.MTT法对照组吸光度值为0.50±0.01,实验组为0.45 ±0.02（P ＜0.05）;对照组划痕愈合率为（47.45±10.05）％,实验组为（88.23±5.45）％ （P ＜0.05）.（2） zebularine作用24h能显著增加E-cadherin mRNA的表达,对照组相对表达量为1.02±0.10,实验组为4.25±0.56（P ＜0.05）.结论 甲基化转移酶抑制剂zebularine可以有效抑制SGC-7901细胞株的增殖和迁移,并提高抑癌基因E-cadherin mRNA的表达.

Objective To study the effect of DNA methylation inhibitor zebularine on the proliferation,migration and the expression of E-cadherin in human gastric carcinoma cell line SGC-7901.Methods Methyl thiazol tetrazolium （MTT） and the wound healing assays were used to evaluate the effect of zebularine on proliferation and migration of SGC-7901 cells.The mRNA expression of E-cadherin was detected by real-time quantitative polymerase chain reaction （Real-time PCR）.Results （1） zebularine at a final concentration of 100 μmol/L significantly inhibited the proliferation （0.50 ± 0.01 vs.0.45 ± 0.02） and migration capacity [（47.45 ± 10.05） % vs.（88.23 ± 5.45） %] of SGC-7901 cells after 24 h （P 〈 0.05）.（2） Zebularine significantly increased the mRNA expression of E-cadherin in SGC-7901 cells after 24 h （1.02 ± 0.10 vs.4.25 ± 0.56,P 〈 0.05）.Conclusion DNA methylation inhibitor zebularine can effectively decrease the proliferation and migration of SGC-7901 cells,and significantlv increase the mRNA expression of E-cadherin at a final concentration of 100 μmol/L after 24 h.