膜型基质金属蛋白酶-2在胃癌组织中的表达与预后的意义

Expression of membrane type-2 matrix metalloproteinase in gastric cancer tissues and its correlation with prognosis

目的 探讨胃癌组织中膜型基质金属蛋白酶-2（MT2-MMP）的表达水平与预后的关系.方法 采用免疫组织化学染色法检测胃癌组织芯片中90例癌组织及配对的癌旁组织中MT2-MMP的表达.采用x2检验分析MT2-MMP表达与临床病理特征的关系;采用Kaplan-Meier法及Log-rank检验分析MT2-MMP表达与总生存期（OS）的关系;拟合多因素Cox模型,采用风险比（HR）及95％可信区间（CI）评价不同临床指标与死亡的相关性.结果 MT2-MMP在胃癌组织中的表达高于癌旁组织（H-score评分均值分别为246和139分,P＜0.01）;胃癌组织中MT2-MMP的表达水平和患者的性别、年龄、肿瘤大小、病理分级、TNM分期无明显相关（P＞0.05）;单因素和多因素回归分析均显示MT2-MMP高表达胃癌患者的死亡风险较低表达组显著增加（HR=2.185,95％ CI：1.175-4.064,P＜0.05;HR=1.997,95％ CI：1.042 -3.830,P＜0.05）.结论 MT2-MMP高表达提示胃癌患者预后较差.

Objective To investigate the expression of membrane type-2 matrix metalloproteinase （MT2-MMP） in gastric cancer and its correlation with prognosis.Methods The expression of MT2-MMP was detected by immunohistochemistry （IHC） in tissue microarrays containing paired tumor and peritumoral tissues from 90 patients who had undergone gastric resection for histologically proven gastric cancer.Chi-square test was utilized to analyze the relationship between MT2-MMP expression and clinicopathological features.Overall survival （OS） of different clinicopathological characteristics was compared using Kaplan-Meier and Log-rank test.The Cox proportional hazards model was performed to estimate hazard ratio （HR） with 95% confidence interval （CI） of the linking strength between the different clinical characteristics,expression of MT2-MMP and death risks.Results The MT2-MMP expression level was higher in tumor tissues than in peritumor tissues （the mean H-scores were 246 and 139 respectively,P 〈 0.01）.No significant correlation was found between the MT2-MMP expression and gender,age,tumor size,pathological stage,TNM stage （all P 〉 0.05）.Both univariate and multivariate analyses demonstrated that high MT2-MMP expression was a negative prognostic indicator of OS （HR =2.185,95% CI：1.175-4.064,P 〈 0.05 ;HR =1.997,95% CI：1.042-3.830,P 〈 0.05,respectively）.Conclusion High expression of MT2-MMP was associated with poor prognosis.