正常甲状腺、结节性甲状腺肿及甲状腺癌新鲜离体组织的红外光谱研究

Fourier transform infrared spectrum of normal thyroid, nodular goiter and thyroid cancer

目的 探讨利用傅里叶变换红外（FTIR）光谱技术术中快速诊断甲状腺疾病的新方法.方法 联合应用衰减全反射（ATR）探头与FTIR光谱仪,测定34例正常甲状腺、56例结节性甲状腺肿及46例甲状腺癌组织的FTIR光谱,用方差分析或非参数检验等方法比较3组光谱10个谱带的峰位、相对峰强及半高宽等29个FTIR参数,找出正常甲状腺、结节性甲状腺肿及甲状腺癌的光谱差异.结果 正常甲状腺、结节性甲状腺肿及甲状腺癌红外光谱在蛋白、核酸、脂类和糖类等多个相关谱带的多个参数表现出显著性差异.通过最小显著差数法（LSD）或Tamhane＇s T2法两两比较,进一步研究了甲状腺癌和结节性甲状腺肿在上述FTIR参数的具体变化趋势,主要有：与核酸相关的1 250/cm处峰的半高宽F1250在3类组织光谱中出现递增的变化趋势,且两两比较差异均有统计学意义.癌组织光谱与另外两种组织比较在1 085/cm处的峰位P1085发生了明显的蓝移（向高波数移动）,相对峰高I1085/I1460也显著升高.半高宽F1460在结节性甲状腺肿中表现出了特异性的缩窄.F1120在结节性甲状腺肿光谱中相较另外两类显著的增宽.结论 正常甲状腺、结节性甲状腺肿及甲状腺癌红外光谱在多个谱带的峰位、相对峰强及半高宽等方面存在显著的差异.FTIR测定有望成为术中甲状腺组织快速定性诊断的有力工具.

Objective To explore a novel diagnostic method of normal thyroid,nodular goiter and thyroid cancer by Fourier transform infrared （FTIR） spectrum intraoperatively.Methods Thirty-six cases of FTIR spectra of freshly removed normal thyroid tissues,56 of the nodular goiter and 46 of thyroid cancer were collected.Twenty-nine FTIR parameters of 10 bands including peak position,relative intensity ratio and full width at half maximum （FWHM） were measured.The data were analyzed by one-way ANOVA.Results Lots of FTIR parameters related to nucleic acid,protein,carbohydrate,and lipid were different in a statistically significant way among the three groups.Further Least-Significant Difference （LSD） or Tamhane＇ s T2 test were performed,and the results showed：F1 250 increased in a pairwise way （P〈0.05）; Compared to other two groups,P1085 moved to the high wave numbers and I1085/I1460 increased significantly in cancer group; F1 460 narrowed while F1 120 widened specifically in goiter group.Conclusion FTIR spectroscopy is a potentially powerful diagnostic tool of thyroid diseases and can be applied intraoperatively in future.