脑膜瘤组织瘤周水肿、新生血管与血管内皮生长因子表达的关系

Relationship of meningioma peritumoral edema, angiogenesis and expressions of vascular endothelial growth factor

目的 探讨脑膜瘤组织中血管内皮生长因子（VEGF）的表达,及其与血管新生及瘤周水肿（PTBE）的关系.方法 选取神经外科手术切除并经病理检查证实的人脑膜瘤液氮冻存标本84例,其中有瘤周水肿48例（水肿组）,无水肿36例（非水肿组）.另外取10例同期本院因颅脑外伤行标准骨瓣减压术的正常脑组织作为对照组.免疫组织化学及Western blot检测组织中VEGF的蛋白表达,通过反转录-聚合酶链反应（RT-PCR）法检测VEGF mRNA表达;免疫组织化学染色检测标本中CD34的表达并判定微血管密度（MVD）,计算瘤周水肿指数（EI）,并分析其与VEGF表达的相关性.结果 水肿组、非水肿组及对照组脑组织中VEGF蛋白表达量不同,分别为1.892 ±0.214、1.168 ±0.218、0.683±0.197,差异有统计学意义（P＜0.05）;水肿组、非水肿组及对照组脑组织中VEGF mRNA的表达量分别为1.232±0.257、1.002 ±0.132、0.702±0.198,差异有统计学意义（P＜0.05）.两两比较水肿组、非水肿组及对照组脑组织中MVD、VEGF蛋白和mRNA表达依次降低,差异有统计学意义（P＜0.05）;水肿组VEGF蛋白的表达与MVD及EI呈正相关（r=0.675、0.635,P＜0.05）.结论 脑膜瘤组织中VEGF的表达增加,可促进脑膜瘤新血管生成,影响PTBE的形成.

Objective To study the relationship of vascular endothelial growth factor （VEGF） expression level with angiogenesis and peritumoral brain edema （PTBE） in meningiomas tissue.Methods 84 cases of meningioma cryopreserved specimens were collected,including 48 cases peritumoral edema （swelling group）,36 cases of edema （swelling of non-group）.Also take 10 cases of normal brain tissue over the same period due to normal brain tissue hospital traumatic brain injury line standard decompressive craniectomy as a control group.VEGF protein expression level were detected by Immunohistochemistry and Western blotting,the expression of VEGF mRNA level were detected by reverse transcriptase-polymerase chain reaction （RT-PCR） assay; expression of CD34 and determination microvascular density （MVD） were detected through immunohistochemical staining,the calculation of peritumoral edema index （EI）,and the correlation with VEGF expression were analyze.Results Edema group,non-edema and control groups differ in brain tissue VEGF protein expression,respectively,1.892 ±0.214,1.168 ±0.218,0.683 ±0.197,the difference was statistically significant （P 〈 0.05） ; edema group,non-edema and control groups expression of VEGF mRNA in brain tissue were 1.232 ±0.257,1.002 ±0.132,0.702 ±0.198,the difference was statistically signigicant （P 〈 0.05）.Conclusion Expression of VEGF was increased in meningioma tissue and may promote angiogenesis meningiomas and affect the formation PTBE.