摘要
利用层析法从小桃儿七中分离得到单体螺甾烷皂苷,利用核磁共振波谱技术鉴定其结构,采用MTT法测试其体外乳腺癌MCF-7细胞株、人宫颈癌Hela细胞株和人肝癌Hep G2细胞株的细胞毒活性,检测其对正常929细胞株的细胞毒活性。结果表明,该单体对人宫颈癌Hela细胞株具有抑制作用(IC50=71.23μmol/L),对正常929细胞株无明显抑制作用。
Monomer spirostanol glycoside was obtained from the herbs of Xiao Tao Er Qi by chromatographic method and was identified by means of NMR spectral method. Its cytotoxic activities against MCF-7 cell lines,Hela cell lines,Hep G2 cell lines and the normal 929 cell lines were evaluated. The results show that the spirostanol glycoside can inhibit Hela cell lines( IC50 values of 71. 23μmol /L) and has no obvious cytotoxic activities against the normal 929 cell lines.
出处
《济南大学学报(自然科学版)》
CAS
北大核心
2015年第1期12-15,共4页
Journal of University of Jinan(Science and Technology)
基金
山东省优秀中青年科学家科研奖励基金(BS2011CL006)
济南大学博士基金(XBS1203)
济南大学科研基金(自然科学类)(XKY1208)
关键词
小桃儿七
铁筷子
螺甾烷皂苷
人宫颈癌细胞株
细胞毒活性
Xiaotaoerqi
Helleborus thibetanus
spirostanol glycoside
Hela cell line
cytotoxic activity