Cica-Beta Test试剂盒检测耐亚胺培南铜绿假单胞菌金属β-内酰胺酶的评估

Evaluation of Cica-Beta Test Kit for Detection of Metallo-β-Lactamase-Producing Pseudomonas Aeruginosa

目的：评估Cica-Beta Test试剂盒对铜绿假单胞菌（PAE）金属β-内酰胺酶（MBLs）检测的临床适用性。方法选取文献[5]中的82株耐亚胺培南 PAE，PAE-MHT法和 Cica-Beta Test试剂盒分别进行 MBLs 检测。结果 PAE-MHT法的敏感度、特异度和准确率分别为84.6％，97.2％和97.6％。Cica-Beta Test试剂盒敏感度、特异度和准确率分别为76.9％，100％和96.3％。PAE-MHT法和Cica-Beta Test试剂盒有非常好的一致性。结论两种方法均适合应用于临床快速筛查非发酵菌产 MBLs。

Objective To evaluate Cica-Beta Test kit for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa （PAE）in the clinical microbiology laboratory.Methods A total of 82 imipenem-resistant PAE clinical isolates from literature[5]was dectected to metallo-β-lactamase （MBLs）by PAE-MHT and Cica-Beta Test kit.Results The sensitivity,specificity and accuracy rate of PAE-MHT was 84.6%,97.2% and 97.6%,and the sensitivity,specificity and accuracy rate of Cica-Beta Test kit was 76.9%,100% and 96.3%,respectively.Two methods had a good consistency.Conclusion Two methods are simple,quick for detecting to metallo-β-lactamase-producing Pseudomonas in clinical laboratories.