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猪传染性胃肠炎病毒spike蛋白C抗原位点的原核表达研究 被引量：2

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摘要 为猪传染性胃肠炎病毒(TGEV)的血清学检测方法的建立提供必要的诊断抗原,通过基因重组的方法,在大肠杆菌表达系统中表达了猪传染性胃肠炎病毒(TGEV)spike蛋白C抗原位点。Western blot试验表明:表达的重组蛋白具有良好的抗原性。研究为TGEV血清学检测方法的建立提供了必要的物质基础。
作者 于天飞 朱可佳 张双 杨怀娇 李赫 闫健哲 张晓琳 陈刚
机构地区 齐齐哈尔大学生命科学与农林学院 东北农业大学生命科学学院
出处 《黑龙江畜牧兽医（下半月）》 CAS 北大核心 2015年第3期72-74,共3页
基金 黑龙江省自然科学基金项目(QC2014C034) 齐齐哈尔大学青年教师科研启动项目(2010k-M08)
关键词 TGEV PRCV spike蛋白(纤突蛋白) C抗原位点 Western BLOT法
分类号 S852.612 [农业科学—基础兽医学] Q786 [生物学—分子生物学]
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参考文献10

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二级参考文献14

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共引文献12

同被引文献7

引证文献2

二级引证文献8

黑龙江畜牧兽医（下半月）
2015年 第3期

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