七种药用植物c4h基因密码子偏好性及聚类分析

Codon Usage Bias and Cluster Analysis of c4h Genes in Seven Medicinal Plants

为了深入研究药用植物基因特性,利用CodonW和SPSS18.0软件对丹参、黄芪、黄芩、忍冬、桑树、野甘草、长春花共7种药用植物c4h基因密码子进行偏好性和聚类分析。结果表明：7种药用植物c4h基因的有效密码子数介于46.1~55.92,密码子使用偏好性弱,5种药用植物倾向于GC结尾的密码子,2种倾向于AT结尾的密码子。7种药用植物经聚类分析分为两大类,长春花与黄芪为一大类,另一大类中,丹参与黄芩为一类,野甘草、桑树和忍冬为一类。

In order to further study genetic features of medicinal plants,coding usage of c4 h genes in medicial plants including Salvia miltiorrhiza,Catharanthus roseus,Scoparia dulcis,Scutellaria baicalensis,Morus alba,Astragalus mongholicus,Lonicera japonica were analyzed by CodonW.Cluster analysis of c4 hgenes codon bias were carried out by SPSS18.0.The result showed that c4 h genes ENc were between 46.1and 55.92 in seven medical plants,they had a weak codon bias,5medicinal plants tended to use codon ending with GC,2medicinal plants tends to use codon ending with AT.Seven medicinal plants were divided into two parts,one part included Catharanthus roseus and Astragalus mongholicus.In the other part,Salvia miltiorrhiza and Scutellaria baicalensis were in a group,Scoparia dulcis,Morus alba and Lonicera japonica were in another group.