香砂参术颗粒中橙皮苷的含量测定

Determination of Hesperidin in the Xiang Sha Shen Shu Granules

目的：建立高效液相色谱法测定香砂参术颗粒中橙皮苷的含量.方法：采用WatersC18色谱柱（250mm×4.6nm,5μm）,流动相为乙腈-0.2％磷酸溶液（21：79）,流速为1.0mL·min-1,检测波长为284nm,柱温30℃.结果：橙皮苷进样量在8μg^80μg（r＝0.999）范围内具有良好的线性关系,平均加样回收率（n＝6）为101.66％.结论：本方法灵敏度高,操作简便,准确可靠,可用于香砂参术颗粒中橙皮苷的含量测定.

Objective： To develop the determination of Hesperidin in the the Xiang Sha Shen Shu Granules by HPLC. Methods： A Waters C18 （250mm× 4.6mm, 5μm） column was used with a mobile phase of acetonitrile--phosphoric acid 0.2% tech grade （21：79） at a flow rate of 1.0mL ·min-1; the wavelength of UV detection was 284nm, and the column temperature was 30℃C. Results： There was a good linear relationship （r= 0.9999） when the injectiou volume of Hesperidin was 8μg-80μg; the average recov- ery （n=6） was 101.66%. Conclusion： This method is highly sensitive, convenient, accurate and reliable, which can be used to determine Hesperidin in the Xiang Sha Shen Shu Granules.