HPLC法测定满山红提取物中多个黄酮成分总量

Determination of Total Contents of Multiple Flavonoids in Extractive of Dried Leaves of Rhododendron Dauricum by HPLC

目的：建立满山红提取物中金丝桃苷、杜鹃素、槲皮素和山奈酚总量的高效液相色谱测定方法。方法：采用高效液相色谱法测定满山红提取物中4种黄酮类成分的总量。结果：金丝桃苷、杜鹃素、槲皮素、山奈酚的线性范围分别为19.248-481.2μg·ml-1（r=0.9999）、7.984-199.6μg·ml-1（r=0.9998）、5.648-141.2μg·ml-1（r=0.9996）、1.408-35.2μg·ml-1（r=0.9998）、平均加样回收率分别为96.5%、98.4%、97.4%、97.5%,RSD分别为1.25%、0.70%、0.71%、0.89%。四批满山红提取物中多个黄酮成分总量分别为3.17%、3.14%、3.06%、3.19%。结论：该方法简便、重复性好、结果准确可靠,可用于满山红提取物的质量控制。

Objective： To establish a method to determine the total contents of Hyperoside, Farrerol, Quercetin and Kaempferol in extractive of dried leaves of Rhododendron Dauricum. Methods： HPLC was adopted for the determination of the total contents of four flavonoids in extractive of dried leaves of Rhododendron Dauricum. Results： The Hyperoside, Farrerol,Quercetin and Kaempferol had good linearity in the range of 19. 248 - 481. 2μg. ml-1（ r = 0. 9999）,7. 984 - 199. 6μg. ml-1（ r=0. 9998）,5. 648 - 141. 2μg. ml-1（ r = 0. 9996） and 1. 408 - 35. 2μg. ml-1（ r = 0. 9998）,the average recoveries were96. 5%,98. 4%,97. 4% and 97. 5% with RSD of 1. 25%,0. 70%,0. 71% and 0. 89% respectively,the total contents of four groups were 3. 17%,3. 14%,3. 06% and 3. 19%. Conclusion： The method is simple,repeatable and accurate,it can be used for quality control of extract from dried leaves of Rhododendro Dauricum.