EMS处理对大白菜种子和幼苗活力的影响及M_2表型变异分析

Effects of EMS Mutagenesis on Seed and Seedling Vigor and Phenotypic Variation of M_2 Population in Chinese Cabbage

大白菜突变体的获得是种质创新的有效途径,也是开展功能基因组研究的材料基础。本研究选取自交亲和、易进行小孢子培养的大白菜自交系‘A03’作为构建突变体库的野生型材料。分析EMS种子处理对M1、M2种子和幼苗活力及生理特性的影响,确定适宜诱变方案并构建突变体库,并针对突变体库M2群体结球期、收获期和生殖生长阶段表型性状变异进行了分析。结果表明,以浓度0.4%的EMS浸泡种子16 h,以及连续对两代种子进行诱变处理,均可以构建大白菜突变体库。突变体库M2群体植株在结球期、收获期和生殖生长阶段表型变异丰富,变异频率分别为21.65%、22.40%和25.65%。

Creating mutants is an effective way for germplasm innovation and provides material for functional genomics research in chinese cabbage. In this study,the inbred line＇ A03＇ of Chinese cabbage was selected as wild genotype for constructing mutant library because of its self-compatibility and easy microspore culture. The influences of EMS treatments on vigor and some physiological property of M1 and m^2seeds and seedlings were investigated in order to determine the appropriate mutagenesis method. Then the mutant library of Chinese cabbage was constructed. The variation in phenotypic traits of m^2 population at heading,harvest,and reproductive growth stages was further investigated and analyzed. The results showed that the most suitable scheme of creating mutants was considered to soak seeds with EMS 0. 4% for 16 h. The way of continuous treatments with EMS on seeds of two generations could be used in constructing mutant library of Chinese cabbage. Within the obtained mutant population,there was wide variation in phenotypic traits of m^2 individuals at heading,harvest,and reproductive growth stage,with mutation frequencies of 21. 65%,22. 40% and 25. 65%,respectively.