基于高蛋白饮食建立鸡痛风性关节炎模型的量效关系研究

A dose-effect relationship study of gouty arthritis chicken model built by high-protein diet

目的:探讨基于高蛋白饮食建立鸡痛风性关节炎模型时饲料蛋白含量与造模效果之间的量效关系。方法:将160只30日龄雄性湘黄鸡随机分为对照组、模型1组、模型2组和模型3组,每组40只,分别以蛋白含量为19.8%、40.19%、50%和55.2%的饲料喂养,连续喂养21 d。分别于造模开始后1、7、14、21 d在每组随机选出10只鸡,分别观察其一般状态、踝关节周径和血尿酸含量;随后处死动物,分离出左侧踝关节滑膜,观察其滑膜组织形态。结果:1一般状态。除造模开始后1 d时外,其余各时点4组一般状态评分比较,组间差异均有统计学意义(F=65.721,P=0.271;F=70.634,P=0.013;F=59.448,P=0.001;F=73.124,P=0.001)。造模开始后7、14、21 d时,对照组的评分均高于模型1组、模型2组和模型3组(P=0.021,P=0.013,P=0.020;P=0.001,P=0.001,P=0.001;P=0.000,P=0.000,P=0.000),模型2组的评分均低于模型1组和模型3组(P=0.031,P=0.025;P=0.001,P=0.001;P=0.000,P=0.000),模型1组与模型3组比较差异均无统计学意义(P=0.125,P=0.112,P=0.141)。2踝关节周径。除造模开始后1 d时外,其余各时点4组踝关节周径比较,组间差异均有统计学意义(F=115.172,P=0.162;F=124.445,P=0.014;F=121.237,P=0.002;F=119.881,P=0.002)。造模开始后7、14、21 d时,对照组的踝关节周径均低于模型1组、模型2组和模型3组(P=0.033,P=0.024,P=0.022;P=0.012,P=0.010,P=0.011;P=0.001,P=0.000,P=0.001),模型2组的踝关节周径均大于模型1组和模型3组(P=0.032,P=0.027;P=0.012,P=0.011;P=0.001,P=0.001),模型1组与模型3组比较差异均无统计学意义(P=0.141,P=0.126,P=0.134)。3血尿酸含量。除造模开始后1 d时外,其余各时点4组血尿酸含量比较,组间差异均有统计学意义(F=35.361,P=0.273;F=52.371,P=0.021;F=56.734,P=0.004;F=48.336,P=0.003)。造模开始后7、14、21 d时,对照组的血尿酸含量均低于模型1组、模型2组和模型3组(P=0.021,P=0.022,P=0.019;P=0.011,P=0.004,P=0.010;P=0.003,P=0.001,P=0.003),模型2组的血尿酸含量均高于模型1组和模型3组(P=0.031,P=0.033;P=0.011,P=0.011;P=0.002,P=0.001),模型1组与模型3组比较差异均无统计学意义(P=0.138,P=0.220,P=0.341)。4滑膜组织形态。除造模开始后1 d时外,其余各时点4组滑膜中血管数量比较,组间差异均有统计学意义(F=26.772,P=0.183;F=32.176,P=0.022;F=33.672,P=0.003;F=41.351,P=0.001)。造模开始后7、14、21 d时,对照组滑膜中血管数量均少于模型1组、模型2组和模型3组(P=0.022,P=0.022,P=0.020;P=0.016,P=0.011,P=0.014;P=0.005,P=0.001,P=0.003),模型2组滑膜中血管数量均多于模型1组和模型3组(P=0.016,P=0.012;P=0.010,P=0.011;P=0.001,P=0.001),模型1组与模型3组比较差异均无统计学意义(P=0.243,P=0.251,P=0.320)。除造模开始后1 d时外,其余各时点4组滑膜中中性粒细胞数量比较,组间差异均有统计学意义(F=32.347,P=0.311;F=43.561,P=0.014;F=42.361,P=0.000;F=51.745,P=0.000)。造模开始后7、14、21 d时,对照组滑膜中中性粒细胞数量均少于模型1组、模型2组和模型3组(P=0.025,P=0.018,P=0.021;P=0.014,P=0.010,P=0.012;P=0.004,P=0.002,P=0.004),模型2组滑膜中中性粒细胞数量均多于模型1组和模型3组(P=0.027,P=0.022;P=0.015,P=0.013;P=0.002,P=0.003),模型1组与模型3组比较差异均无统计学意义(P=0.220,P=0.351,P=0.117)。结论:通过高蛋白饮食诱导可获得鸡痛风性关节炎模型,其中以蛋白含量为50%的饮食造模效果最好。

Objective： To explore the dose-effect relationship between feedstuff protein content and gouty arthritis chicken models builtby high-protein diet. Methods： One hundred and sixty 30-day-old male XIANGHUANG chickens were randomly divided into control group,model group 1,model group 2 and model group 3,40 cases in each group. The chickens were fed for consecutive 21 days with feedstuff which protein content was 19. 8%,40. 19%,50% and 55. 2% respectively. Ten chickens were randomly selected from each group at 1,7,14 and 21 days after the beginning of modeling respectively; and the general state,ankle circumference and blood uric acid levels were detected. Then the chickens were executed,and the left ankle synovium were separated for observing the synovial tissue morphology. Results：There was statistical difference in general state scores between the 4 groups except at one day after the beginning of modeling（ F = 65. 721,P = 0. 271; F = 70. 634,P = 0. 013; F = 59. 448,P = 0. 001; F = 73. 124,P = 0. 001）. The scores of control group were higher than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling（ P = 0. 021,P = 0. 013,P = 0. 020;P = 0. 001,P = 0. 001,P = 0. 001; P = 0. 000,P = 0. 000,P = 0. 000）; and the scores of model group 2 were lower than that of model group1 and model group 3（ P = 0. 031,P = 0. 025; P = 0. 001,P = 0. 001; P = 0. 000,P = 0. 000）; there was no statistical difference in general state scores between model group 1 and model group 3（ P = 0. 125,P = 0. 112,P = 0. 141）. There was statistical difference in ankle circumference between the 4 groups except at one day after the beginning of modeling（ F = 115. 172,P = 0. 162; F = 124. 445,P = 0. 014; F =121. 237,P = 0. 002; F = 119. 881,P = 0. 002）. The ankle circumference of control group were less than that of model group 1,model group2 and model group 3 at 7,14 and 21 days after the beginning of modeling（ P = 0. 033,P = 0. 024,P = 0. 022; P = 0. 012,P = 0. 010,P =0. 011; P = 0. 001,P = 0. 000,P = 0. 001）; and the ankle circumference of model group 2 were greater than that of model group 1 and model group 3（ P = 0. 032,P = 0. 027; P = 0. 012,P = 0. 011; P = 0. 001,P = 0. 001）; there was no statistical difference in ankle circumference between model group 1 and model group 3（ P = 0. 141,P = 0. 126,P = 0. 134）. There was statistical difference in blood uric acid levels between the 4 groups except at one day after the beginning of modeling（ F = 35. 361,P = 0. 273; F = 52. 371,P = 0. 021; F = 56. 734,P =0. 004; F = 48. 336,P = 0. 003）. The blood uric acid levels of control group were lower than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling（ P = 0. 021,P = 0. 022,P = 0. 019; P = 0. 011,P = 0. 004,P = 0. 010; P =0. 003,P = 0. 001,P = 0. 003）; and the blood uric acid levels of model group 2 were higher than that of model group 1 and model group 3（ P = 0. 031,P = 0. 033; P = 0. 011,P = 0. 011; P = 0. 002,P = 0. 001）; there was no statistical difference in blood uric acid levels between model group 1 and model group 3（ P = 0. 138,P = 0. 220,P = 0. 341）. There was statistical difference in the number of blood vessels in synovium between the 4 groups except at one day after the beginning of modeling（ F = 26. 772,P = 0. 183; F = 32. 176,P = 0. 022; F =33. 672,P = 0. 003; F = 41. 351,P = 0. 001）. The number of blood vessels in synovium of control group was fewer than that of model group1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling（ P = 0. 022,P = 0. 022,P = 0. 020; P = 0. 016,P =0. 011,P = 0. 014; P = 0. 005,P = 0. 001,P = 0. 003）; and the number of blood vessels in synovium of model group 2 was more than that of model group 1 and model group 3（ P = 0. 016,P = 0. 012; P = 0. 010,P = 0. 011; P = 0. 001,P = 0. 001）; there was no statistical difference in the number of blood vessels in synovium between model group 1 and model group 3（ P = 0. 243,P = 0. 251,P = 0. 320）. There was statistical difference in the number of neutrophils in synovium between the 4 groups except at one day after the beginning of modeling（ F =32. 347,P = 0. 311; F = 43. 561,P = 0. 014; F = 42. 361,P = 0. 000; F = 51. 745,P = 0. 000）. The number of neutrophils in synovium of control group was fewer than that of model group 1,model group 2 and model group 3 at 7,14 and 21 days after the beginning of modeling（ P = 0. 025,P = 0. 018,P = 0. 021; P = 0. 014,P = 0. 010,P = 0. 012; P = 0. 004,P = 0. 002,P = 0. 004）; and the number of neutrophils in synovium of model group 2 was more than that of model group 1 and model group 3（ P = 0. 027,P = 0. 022; P = 0. 015,P = 0. 013; P =0. 002,P = 0. 003）; there was no statistical difference in the number of neutrophils in synovium between model group 1 and model group 3（ P = 0. 220,P = 0. 351,P = 0. 117）. Conclusion： The gouty arthritis chicken model can be built by high-protein diet induction,and the diet which protein content is 50% has the best induction effect.