糖原合酶激酶3β对少突胶质前体细胞分化的影响

The critical role of GSK3β in oligodendrocyte development

目的探讨抑制糖原合酶激酶3β活性对少突胶质前体细胞分化为成熟的少突胶质细胞的影响。方法原代分离培养少突胶质前体细胞,采用免疫印迹法和免疫荧光染色技术检测糖原合酶激酶3β在少突胶质前体细胞和少突胶质细胞中的表达。利用1.5 m M氯化锂抑制糖原合酶激酶3β96 h,采用免疫印迹法和免疫荧光染色技术检测糖原合酶激酶3β对少突胶质前体细胞发育的影响。结果随着少突胶质前体细胞发育为少突胶质细胞,总的糖原合酶激酶3β的表达量不变,而非活性形式的糖原合酶激酶3β(磷酸化的糖原合酶激酶3β)减少,说明活性糖原合酶激酶3β增加。加入氯化锂后,磷酸化的糖原合酶激酶3β显著增加,表明活性糖原合酶激酶3β减少,并且成熟的少突胶质细胞的标记物髓鞘碱性蛋白的信号显著减弱,表明抑制糖原合酶激酶3β的活性后明显阻碍了少突胶质前体细胞正常的分化过程。结论糖原合酶激酶3β在少突胶质前体细胞分化为成熟的少突胶质细胞的过程中起着重要的作用。

Objective To observe the effect of inhibiting GSK3β activity on the differentiation of oligodendrocyte precursor cells（ OPCs） into the oligodendrocytes（ OLs）. Methods OPCs were successfully cultured and the expression of GSK3β in OPCs and OLs were detected by western blot and immunocytochemistry techniques. OPCs were treated with 1. 5m M Li Cl for 96 h,and the GSK3β and MBP levels were measured by western blot and immunocytochemistry techniques. Results As OPCs differentiated into OLs,the total GSK3β（ t GSK3β） level didn’t change,but non- activated GSK3β（ p GSK3β） level remarkably increased indicating that activated GSK3β decreased in OLs compared with OPCs. When treated with Li Cl,p GSK3β increased showing that activated GSK3β decreased. In the mean time,the signal of MBP,one of the OLs maker decreased. Conclusion GSK3β plays an important role in the process of OPCs differentiation into OLs.