摘要
根据香草醛-高氯酸显色反应后样品吸光度的差异,用Box-Behnken响应面法优化了海马中甾醇的提取条件。主要确定了提取温度、提取时间及料液比3个因素对甾醇提取效果的影响。并以胆固醇为标准品,测定了海马中总甾醇的含量。结果表明:提取温度为79℃,提取时间为7.7 h,料液比为80 ml/g时,海马中的总甾醇提取量最高,提取量为5.17 mg/g。通过高效液相分析,初步分离海马总甾醇提取物,为海马中甾醇成分的提取和分离提供了初步的依据。
In the present work, we optimized the extracting conditions of total sterol in Hippocampus kuda by Box-Behnken response surface method according to the difference in absorbance of vanillin perchloric acid color reaction. Three factors associated with extraction effect were determined , including extraction temperature, extraction time and ratio of material to liquid. The content of total sterol in the hippocampus was also determined with a standard of cholesterol. The results showed that the highest total sterol extraction amount was obtained to 5.17 mg/g when the extraction temperature was at 79℃, extraction time at 7.7 h, and the ratio of solid to liquid at 80 mL/g. The purified sample was further identified by high performance liquid chromatography （HPLC）. This paper provided a preliminary basis for the extraction and separation of the sterol composition in Hippocampus kuda.
出处
《食品研究与开发》
CAS
北大核心
2015年第1期40-45,共6页
Food Research and Development
基金
国家海洋经济创新发展区域示范战略性新兴产业发展专项项目
宁波市科技计划重大专项(宁波市农业科技攻关项目)(2014C10006)
关键词
海马
总甾醇
紫外分光光度法
高效液相
响应面
Hippocampus kuda
total steroid
UV spectrophotometry
high performance liquid chromatography （HPLC）
response surface method
