新疆昆仑雪菊总黄酮含量测定方法研究

Study on Determination of Total Flavonoids Content in Xinjiang Kunlun Snow Chrysanthemum

以芦丁为标准品,对Na NO2-Al(NO3)3-Na OH法和Al Cl3法测定新疆昆仑雪菊总黄酮含量的结果作了比较。结果表明:Na NO2-Al(NO3)3-Na OH法的测定结果比Al Cl3法对于高原雪菊和平原雪菊分别约高19.76 mg/m L和9.34 mg/m L,Al Cl3法适宜酸度为p H>5.5。邻苯二酚干扰实验表明,平均误差为0.019 mg/L,随着邻苯二酚量的增加,测得值无上升趋势。Al Cl3法的线性方程为:A=0.000 638+0.862 3C,r2=0.999 2。因此,对含酚类、酸类较多的昆仑雪菊应采用三氯化铝法测定其总黄酮的含量。

With rut in as the standard substance, comparing the NaNO2-Al （NO3）3-NaOH determination of total flavonoids content in Xinjiang Kunlun snow chrysanthemum method and AlCl 3 method. The results showed that：NaNO2-Al（NO3）3-NaOH assay results than the AlCl3 method for the Plateau snow chrysanthemum and plain snow chrysanthemum was approximately 19.76 mg/mL and 9.34 mg/mL, AlCl3 acidity was pH＆gt;5.5. The experiment showed that the catechol interference, the average error was 0.019 mg/L, with the increase of the measured value of catechol, no upward trend. Linear equation AlCl3 method was A=0.000 638＋0.862 3C,r2=0.999 2. Therefore, determination of the total flavonoids of phenols, acids more Kunlun snow chrysanthemum should be adopted AlCl3 method.