密封加热法提取沙棘果渣中异鼠李素

Extraction Isorhamnetin from Marc of Hippophae rhamnoides L. with Seal Heating Method

采取密封加热的方法对从沙棘果渣中提取异鼠李素的工艺进行研究。采用单因素实验对提取时间、提取次数、提取温度、料液比及提取溶剂进行筛选,之后对提取条件进行正交验证实验。实验确定了密封加热法提取沙棘果渣中异鼠李素的最佳工艺为:55℃条件下用V乙酸乙酯∶V95%乙醇=4∶6的提取液水浴加热2次,每次3 h,料液比为1∶20(g∶m L)。

Extraction isorhamnetin from marc of Hippophae rhamnoides L. with seal heating method were studied. Single factor experiment was used to select extraction time, extraction times, extraction temperature, solid-liquid ratio and extraction solvent. Then the orthogonal verification experiment was used. The best processes of extraction isorhamnetin from marc of Hippophae rhamnoides L. with seal heating method were as follows：under temperature 55℃, Vethyl acetate∶V95%ethyl alcohol were 4∶6 as extraction solvent, extraction time 3 h, material to liquid ratio 1∶20（g∶mL）, and extraction times 2.