摘要
采用30%的乙腈水(体积比)为稀释液,建立了样品直接稀释、高效液相色谱(HPLC)分离、示差折光检测器检测(RID)快速测定鲜橙中的果糖、葡萄糖、蔗糖、麦芽糖的可靠方法。该方法以保留时间(Rt)定性,外标法定量;在0.05 mg/m L^5 mg/m L的浓度范围内,标准曲线有良好的线性关系,相关系数大于0.999 5;样品在5.0、10.0、25.0 g/kg3个加标水平内的回收率范围为95%~101%(n=10),相对标准偏差小于5.0%;方法的定性检出限(LOD)为0.05 g/kg、定量检出限(LOQ)为0.15 g/kg,该方法具有简便、快速、灵敏的特点。应用本方法对产自四川、云南、湖北、重庆等7个产地的18个不同品种的鲜橙进行了4种糖含量的检测,结果表明4种还原糖含量因品种、产地不同而有差异。
A method was developed for simultaneous determination of fructose,glucose,sucrose and maltose in fresh orange. The sample was diluted directly by acetonitrile-H2O （30%, v/v）, and then analyzed by high-performance liquid chromatography （HPLC） with refractive index detector （RID）. The method for qualitative analysis was based on retention time. And external standard method was used for calculation. Good linear relationship was achieved in the range of 0.05 mg/mL-5 mg/mL for the 4 components with correlation coefficients above 0.999 5. The recoveries of the method ranged from 95%to 101% （n=10） with the relative standard deviations （RSDs） below 5.0 % at 3 spiked levels of 5.0, 10.0, 25.0 g/kg. The limit of detection （LOD） of this method is 0.05 g/kg, while the limit of quantification （LOQ） was 0.15 g/kg. This is a simple, rapid, sensitive method for detection fructose,glucose,sucrose and maltose. 18 kinds of oranges from 7 fields （Si Chuan, Yun Nan, Hu Bei, Chongqing et al） were analyzed by the method and the results showed the content of fructose,glucose,sucrose and maltose depended on the varieties and field of orange.
出处
《食品研究与开发》
CAS
北大核心
2015年第2期114-117,共4页
Food Research and Development
基金
国家质检总局公益性行业科研专项(201310146)
