凉茶中DNA提取方法的建立

DNA Extraction Methods of Herbal Tea

以凉茶植物源性饮品为研究对象,比较了1种硅胶柱法商品试剂盒和传统CTAB法提取凉茶食品基因组DNA的效果;通过紫外分析法和实时荧光定量PCR法对所提取的DNA进行检测,比较所得DNA的浓度和纯度,以及对下游检测的适用性。结果表明,与传统CTAB法相比,研究所建立的方法提取到的DNA无PCR抑制剂,浓度和纯度能满足后续PCR检测要求。

Two current DNA extraction methods for processed foods of herbal tea were compared in the study. These methods included one kind of QIAquick R Spin Columns DNA extraction kits and CTAB method. The extracted DNA were detected to compare concentration , purity and suitability to posterior detection by UV analysis, fluorescence quantification PCR. The results showed that the purity of and concentration extracted DNA from herbal tea was without PCR inhibitor by our established method and could meet the requirement of posterior PCR detection.