高效液相法检测细胞内嘌呤含量研究

Study of detection of purine cellular by HPLC method

目的:改进细胞收集方法以提高高效液相法检测细胞内嘌呤的效率。方法:采用高效液相法检测原位和传统两种收集方法获得的细胞质。结果:原位收集获得的MCF-7细胞质中黄嘌呤,次黄嘌呤,腺嘌呤浓度均高于传统收集细胞法获得的MCF-7细胞质相应的嘌呤浓度。结论:与传统的细胞收集法相比,原位细胞收集法更能得到含量较高的嘌呤。

Objective： To improve the method of cell collection for promotion efficiency of detection purine cellular by HPLC method. Methods： HPLC method,is adopted to detect cytoplasm collected by in situ and tradition- al methods. Results： The concentrations of xanthine, hypoxanthine and adenine collecting by in situ method were more than that by tradition method. Conclusion： Compared with the tradition collection method, in situ collection method was more effective.