摘要
研究黄连解毒汤全方及其拆方有效部位群主要成分溶出变化规律。采用HPLC方法分析全方及不同拆方的有效部位群,色谱柱为Agilent TC-C18(4.6 mm×250 m,5μm),以乙腈(A)-0.2%磷酸水B)为流动相,梯度洗脱,体积流量1.0 m L/min,检测波长为260 nm。黄连解毒汤经大孔树脂50%醇洗脱后能使方中主要成分含量增加。本实验为黄连解毒汤新药研究提供参考。
The variation of components of effective fractions in Huanglianjiedu decoction and its disassembled prescription were studied. Each sample was separated by Agilent TC- C18（ 4. 6 mm × 250 mm,5 μm）. Mobile phase was composed of acetonitrile and water（ with 0. 2% phosphoric acid）,in a gradient elution manner,the flow rate was1. 0 m L / min and the etection wavelength was 260 nm. Throughmacroporous resin elution by 50% alcohol can increase the dissolution of components in Huanglianjiedu decoction. A reference for research the new drugs of Huanglianjiedu decoction was provided.
出处
《广州化工》
CAS
2015年第5期66-67,94,共3页
GuangZhou Chemical Industry
基金
国家自然科学基金项目(81303205)
