超声波-双水相萃取印楝素工艺的研究

The study of ultrasound-aqueous two-phase extraction process of azadirachtin

采用超声波耦合乙醇-硫酸钠双水相提取印楝素,将超声功率密度,超声时间,超声辐射方式作为其影响因素,并分别进行单因素实验和正交实验对其进行研究,最终确定了超声波双水相提取印楝素的最佳工艺。最佳工艺条件为:功率密度为2.4 w/cm2,时间为30 min,辐射方式为7s/10s。并且影响印楝素超声提取率的主次因素为功率密度>辐射方式>超声时间,最优条件下印楝素的提取率为0.3200%。

In this paper,the method of ultrasonic assisted double water phase is used to extract azadirachtin. In order to obtain the best technology of extracting azadirachtin, many influence factors are studied, such as ultrasonic power density, action time and the way of radiation method. Meanwhile, the single factor and orthogonal experiments are performed separately to investigate the extraction of azadirachtin. Finally, the optimum conditions of extracting azadirachtin is gained when the ultrasonic power density, action time and radiation mode are 2.4w/cm2, 30 min and 7s/10 s respectively. The influence degrees from strong to weak are in order caused by ultrasonic power density, radiation mode and ultrasonic action time. The extracted yield of azadiraehtin reaches 0.3200% under optimal conditions.