摘要
目的研究在白藜芦醇(Res)抑制胃癌细胞增殖过程中,胰岛素样生长因子结合蛋白-3(IGFBP-3)表达的变化。方法噻唑蓝(MTT)法测定Res对BGC-823细胞增殖的抑制程度,实时荧光定量聚合酶链反应(qRT-PCR)和Western blot技术检测Res处理后BGC-823细胞中IGFBP-3表达变化,siRNA技术沉默IGFBP-3基因表达,MTT法观察Res对BGC-823细胞增殖的影响,流式细胞技术测定各组细胞凋亡率。结果 Res能够抑制BGC-823生长,经20,40,80,160μmol·L-1Res处理后,细胞存活率分别为(82.35±10.65)%,(74.30±12.36)%,(62.80±14.66)%,(50.75±11.14)%。Res刺激后,IGFBP-3表达水平升高,与对照组比较,IGFBP-3 mRNA水平增高2.96倍(P<0.05),IGFBP-3蛋白水平变化与其mRNA一致。siRNA技术沉默IGFBP-3表达后,160μmol·L-1Res刺激BGC-823细胞,细胞存活率下降(78.5±9.86)%,但高于同浓度下Res刺激的未经IGFBP-3沉默的BGC-823细胞(P<0.05)。IGFBP-3沉默后,160μmol·L-1Res处理后BGC-823细胞凋亡率(20.13±9.12)%,明显低于未经IGFBP-3沉默的BGC-823细胞[(35.48±11.12)%],且差异有统计学意义(P<0.05)。结论 Res可以抑制BGC-823细胞增殖,促进其凋亡。该机制涉及IGFBP-3高表达。
Objective To study the expression of insulin like growth factor binding proteins 3 （IGFBP-3） during inhi-bition of resveratrol （Res） on cell proliferation. Methods The inhibitory effect of Res on BGC-823 cells was determined by MTT method; Real-time qRT-PCR and western blot were applied to detect the expression of IGFBP-3 in Res-treated BGC-823 cells. In addition, cytometry was used to determine the proliferation and apoptosis of Res-treated BGC-823 after knockdown of IG-FBP-3 by siRNA. Results Upon Res （20,40, 80 and 160 μmol · L^-1 ） treatment,the viability of BGC-823 cells was （82. 35±10. 65）% ,（74. 30±12. 36）% ,（62. 80±14. 66）% and （50. 75±11. 14）% , respectively. The mRNA and protein ex-pression of IGFBP-3 elevated as high as 2. 96-fold compared to the control group （P〈0. 05）. The cell viability of BGC-823 cells with IGFBP-3 knockdown was significantly higher than that of the wild type （ P 〈 0. 05 ） only at high Res concentration （160 μmol·L^-1 ）. Meanwhile,IGFBP-3 knockdown led to a significant decrease on cell apoptotic rate by Res （160 μmol·L^-1 ） [（20. 13±9. 12）% vs （35. 48±11. 12）% ,P〈0. 05）]. Conclusion Res can inhibit BGC-823 cell proliferation and promote cell apoptosis, the underlying mechanism of which may be related to the overexpression of IGFBP-3 in BGC-823 cells.
出处
《医药导报》
CAS
2015年第3期317-321,共5页
Herald of Medicine
