摘要
以加替沙星为模板分子,采用溶胶-凝胶分子印迹技术,合成具有分子识别作用的新型有机-无机杂化分子印迹聚合物,对其进行吸附性能研究证明了印迹聚合物对加替沙星的专一吸附性能。以该印迹聚合物为固相萃取材料填充固相萃取柱,优化固相萃取条件,结合超高效液相色谱法,对牛奶样品进行检测。建立了分子印迹固相萃取方法,分离富集并检测牛奶中的加替沙星,其回收率为79.87%,富集倍数为38,高于市售C18柱,具有一定的实际应用价值。
A new molecularly imprinted organic-inorganic hybrid polymer was prepared by sol-gel process in aqueous phase system based on the interaction of ion pair and hydrophobic between gatifloxacin（ GTFX） and the functional monomer,using GTFX as the template molecular. The adsorption property of MIP was studied. The results showed that MIP has specific adsorption property for GTFX. The MIP as solid phase extraction（ SPE） material was filled in solid phase extraction column. The GTFX in milk samples was tested with Ultra Performance Liquid Chromatography（ UPLC） after optimization of solid-phase extraction conditions. A molecularly imprinted solid phase extraction（ MISPE） method was established for separation and enrichment of GTFX in milk. The results showed the recovery rate of MISPE was 79. 87%,which was great than commercial C18 column.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2015年第3期262-267,共6页
Chemical Research and Application
基金
黑龙江省教育厅项目(12531757)资助
精细化工黑龙江省高校重点实验室项目(JX201208)资助
关键词
分子印迹技术
加替沙星
固相萃取
molecularly imprinted technology
gatifloxacin
solid phase extraction
